Hieff UNICON™ Hotstart Direct DNA Polymerase, 5 U/μL -10717ES

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SKU: 10717ES72

Size: 250 U
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Description

Hieff Unicon Hotstart Direct Taq DNA Polymerase is a hotstart DNA polymerase that is resistant to blood and other inhibitors. The product is blocked by antibodies and has good amplification sensitivity and specificity. After heating  at the pre-denaturation temperature, the antibody is completely inactivated and the DNA polymerase activity is released. The amplification induced by nonspecific annealing of primers could be effectively inhibited by using the hotstart Taq enzyme.

Components

Components

No.

 

Name

10717ES72 (250 U)

10717ES80 (1000 U)

10717ES92 (10 KU)

10717ES93 (25 KU)

10717ES94 (50 KU)

 

10717

Hotstart

D-Taq (5 U/μL)

 

50 μL

 

200  μL

 

1 mL× 2

 

1 mL× 5

 

10 mL

Storage

The product should be stored at -20°C for 2 years.

Instructions

1.  Reaction Composition

 

Components

Volume (μL)

Final Concentration

2 × Buffera

25

1 ×

Primer/Probe Mixb

X

0.1 -0.5 μM

Hotstart D-Taq (5 U/μL)c

1.2

0.12 U/μL

Template DNAb

X

0.1-100 ng

ddH2O

to 50

-

Note:Be sure to mix well before use, avoid excessive bubbles caused by violent vibration.

a) According to the specific experimental application, it is needed to prepare the corresponding reaction buffer. If a basic buffer (10×) is required, Cat#11374 is recommended.

b) The amount of DNA and the concentration of probes or primers are recommended concentrations. The optimal concentration can be adjusted according to the specific experimental conditions.

c) The amount of enzyme can be adjusted according to the experimental application.

2.  Optimized Cycling Protocol

 

 

Reaction stage

Temperature

Time

Cycle

1

Initial denaturation

95°C

5 min

1

 

2

Amplification reaction

95°C

15 sec

 

45 cycles

60°Ca

30 secb

Note:

a) Amplification reaction: The temperature is adjusted according to the Tm value of the designed primers.

b) Fluorescence signal acquisition: Please set the experimental procedure according to the requirements of the instrument manual.

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