Hieff Unicon™ Purepro Ⅰ TaqMan qPCR Master Mix (UDG plus)-11853ES

YeasenSKU: 11853ES03

Size: 1 mL
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Description

Hieff Union™ Purepro ITaqMan qPCR Master Mix (UDG plus) is a fluorescent quantitative qPCR reagent that uses the company's next-generation antibody method to heat start Taq enzyme. The enzyme used has undergone a specific purification process to remove residual host E. coli DNA and some common environmental bacteria DNA. The 2x TaqMan qPCR Mix pre mixed reagent includes reaction buffer and enzyme mix, already containing Mg2+  and dNTP, and adding factors that effectively inhibit non-specific PCR amplification and improve the efficiency of multiple qPCR reactions, which can ensure primer efficiency while conducting up to four reactions. This product contains UDG enzyme, which can effectively prevent the risk of aerosol pollution.

Components

Components No.

Name

11853ES03

11853ES06

11853ES08

11853ES20

11853ES60

11853

2×TaqMan qPCR Mix

1 mL

5×1 mL

5 mL

20 mL

100 mL


Specifications

Hot Start

Built-in hot start

Detection method

Primer-probe detection

PCR method

qPCR

Polymerase

Taq DNA polymerase

Type of sample

DNA

 

 

 

 

 

 

Application equipment

Applied Biosystems: 5700, 7000, 7300, 7700, 7900HT Fast, StepOne™ , StepOne Plus

™, 7500, 7500 Fast, ViiA™7, QuantStudio™ 3 and 5, QuantStudio™ 6,7,12k Flex;

Bio-Rad: CFX96, CFX384,  iCycler iQ,  iQ5,  MyiQ,  MiniOpticon,  Opticon,  Opticon  2,

Chromo4;

Eppendorf: Mastercycler ep realplex, realplex 2 s;

Qiagen: Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000;

Roche Applied Science: LightCycler 480, LightCycler 2.0; Lightcycler 96;

Stratagene: MX3000P™, MX3005P™, MX4000P™;

Thermo Scientific: PikoReal Cycler; Cepheid: SmartCycler; Illumina: Eco qPCR.

Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 1 year.

Instructions

1.Reaction System

Components

Volume (μL)

Final Concentration

2×  Hieff UniconTM  Universal TaqMan multiplex qPCR master mix

12.5

Primer mix (10  μmol/L)

x

0.1-0.5  μmol/L

Probe mix (10  μmol/L)

x

50-250 nmol/L

Rox reference dye

0.5

Template DNA/cDNA

1-10

-

ddH2O

up to 25

-

*The primer concentration can be adjusted appropriately according to the experimental requirements.

2.  Reaction program

 

Cycle step

Temp.

Time

Cycles

Initial denaturation

95℃

5 min

1

Denaturation

95℃

15 s

 

45

Annealing/Extension

60℃

30 s

*The annealing / extension temperature can be adjusted appropriately according to the experimental requirements.

Notes

Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety!

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