Description
dsDNA BR Assay Kit is a simple, sensitive, accurate and wide range fluorescence quantification kit for double-stranded DNA (dsDNA BR) with good linearity in the range of 2~1000 ng. The kit contains fluorescence detection reagent, buffer and related dsDNA BR standards. Before use, the fluorescence detection reagent is diluted into working solution with buffer, then the dsDNA sample to be measured is added, a standard curve is made, and readings are performed using a fluorescent enzyme marker or Qubit® fluorometer. The product has good tolerance to conventional contaminants such as proteins and salts.
Components
|
Components No. |
Name |
Concentration |
12643ES60 (100T) |
12643ES76 (500T) |
|
12643-A |
dsDNA BR Reagent |
200×concentrate in DMSO |
250 μL |
1.25 mL |
|
12643-B |
dsDNA BR Buffer |
Not applicable |
50 mL |
250 mL |
|
12643-C |
dsDNA BR Standard 1 |
0 ng/μL in TE buffer |
1 mL |
5×1 mL |
|
12643-D |
dsDNA BR Standard 2 |
100 ng/μL in TE buffer |
1 mL |
5×1 mL |
Specifications
|
Assay |
dsDNA Quantitation |
|
Excitation/Emission |
485/530 |
|
For Use With (Equipment) |
Qubit® Fluorometer |
|
No. of Reactions |
100T/500T |
|
Product Line |
Qubit Quantitation |
|
Quantitation Range |
2 ng /μL to 2000 ng/μL |
|
Sample Volume |
1 μL to 20 μL |
|
Detection Method |
Fluorescent |
Storage
2~8°C storage, away from light, valid for six months.