D-Luciferin, Sodium Salt D -40901ES

YeasenSKU: 40901ES02

Size: 500 mg
Sale price$845.00

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D-luciferin is a common substrate for Luciferase and is widely used throughout biotechnology, especially in vivo imaging technology.  The mechanism of action is that luciferin (the substrate) is oxidized to emit light in response to ATP and luciferase (see figure below). It's produced when there's too much luciferin The light quantum number was positively correlated with the concentration of luciferase. Plasmids carrying luciferase encoding gene (Luc) were transfected into cells and introduced into study animals such as rats and mice In vivo, fluorescein is then injected and changes in light intensity are detected using bioluminescence imaging (BLI) to monitor disease progression or drug efficacy in real time and so on. ATP's influence on the reaction system can also be used to indicate energy or vital signs according to changes in bioluminescence intensity.

D-luciferin is also commonly used in in vitro studies, including luciferase and ATP levels analysis; Reporter gene analysis; High-throughput sequencing and various contamination tests. There are currently three product forms: D-luciferin (free acid), D-luciferin salts (sodium and potassium salts). The main difference lies in solubility: the former is water-soluble and the solubility of buffer system is weak, except soluble in weak bases such as low concentration NaOH and KOH solutions, soluble in methanol and DMSO; The latter is easily soluble in water or buffer, easy to use, solvent non-toxic, especially suitable for in vivo experiments. As a solution, there is no substantial difference between the three products in the vast majority of applications.  


  • No radiation, almost harmless to living organisms.
  • Bioluminescence, no excitation light source.
  • So sensitive, you can detect it in a few hundred cells.
  • Good penetration, 3-4cm tissue depth can still be detected.
  • High signal-to-noise ratio, strong fluorescence signal and good anti-interference.


  • In the tumorigenesis experiment in nude mice, the tumor growth was observed without invasion in real time, without tumor stripping measurement.
  • To test the effect of the administration on tumor growth or metastasis, the fluorescein substrate can be completely eliminated within 3 hours, without interference to the drug experiment.
  • The localization and distribution of foreign cells in animals were detected.
  • The target gene or promoter of the target gene is fused to the luciferase gene to detect changes in gene expression during drug treatment or disease progression.
  • Monitoring stem cell transplantation, survival and proliferation; Trace the distribution and migration of stem cells in vivo.


English synonym (S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid sodium salt; D-Luciferin firefly, sodium salt monohydrate;
CAS NO. 103404-75-7
Formula NaC11H7N2O3S2·H2O
Molecular weight 320.32 g/mol
Appearance Light yellow powder
Solubility Solube in water(100 mg/mL)
Purity (HPLC) ≥95%


Components No. Name 40901ES01 40901ES02 40901ES03 40901ES08 40901ES10
40901 D-Luciferin, Sodium Salt 100mg 500 mg 1g 5g 10g


The product should be stored at -15℃ ~ -25℃ for 1 year.


Citations & References:

[1] Guo Y, Guo Y, Chen C, et al. Circ3823 contributes to growth, metastasis and angiogenesis of colorectal cancer: involvement of miR-30c-5p/TCF7 axis. Mol Cancer. 2021;20(1):93. Published 2021 Jun 25. doi:10.1186/s12943-021-01372-0(IF:27.401)

[2] Wang Z, Yu L, Wang Y, et al. Dynamic Adjust of Non-Radiative and Radiative Attenuation of AIE Molecules Reinforces NIR-II Imaging Mediated Photothermal Therapy and Immunotherapy. Adv Sci (Weinh). 2022;9(8):e2104793. doi:10.1002/advs.202104793(IF:16.806)

[3] Liu Q, Sheng Z, Cheng C, et al. Anesthetic Propofol Promotes Tumor Metastasis in Lungs via GABA<sub>A</sub> R-Dependent TRIM21 Modulation of Src Expression. Adv Sci (Weinh). 2021;8(18):e2102079. doi:10.1002/advs.202102079(IF:16.806)

[4] Zhou M, Zhang J, Shen J, et al. Hydrogen sulfide-linked persulfidation of ABI4 controls ABA responses through the transactivation of MAPKKK18 in Arabidopsis. Mol Plant. 2021;14(6):921-936. doi:10.1016/j.molp.2021.03.007(IF:13.164)

[5] Wang J, Wang C, Li Y, et al. Potential of peptide-engineered exosomes with overexpressed miR-92b-3p in anti-angiogenic therapy of ovarian cancer. Clin Transl Med. 2021;11(5):e425. doi:10.1002/ctm2.425(IF:11.492)

[6] Mao Z, Wei X, Li L, et al. Arabidopsis cryptochrome 1 controls photomorphogenesis through regulation of H2A.Z deposition. Plant Cell. 2021;33(6):1961-1979. doi:10.1093/plcell/koab091(IF:11.277)

[7] Xu P, Chen H, Li T, et al. Blue light-dependent interactions of CRY1 with GID1 and DELLA proteins regulate gibberellin signaling and photomorphogenesis in Arabidopsis. Plant Cell. 2021;33(7):2375-2394. doi:10.1093/plcell/koab124(IF:11.277)

[8] Lin S, Wen Z, Li S, et al. LncRNA Neat1 promotes the macrophage inflammatory response and acts as a therapeutic target in titanium particle-induced osteolysis. Acta Biomater. 2022;142:345-360. doi:10.1016/j.actbio.2022.02.007(IF:8.947)

[9] Zhang Z, Zeng D, Zhang W, et al. Modulation of oxidative phosphorylation augments antineoplastic activity of mitotic aurora kinase inhibition. Cell Death Dis. 2021;12(10):893. Published 2021 Sep 30. doi:10.1038/s41419-021-04190-w(IF:8.469)

[10] Wang L, Tong X, Zhou Z, et al. Circular RNA hsa_circ_0008305 (circPTK2) inhibits TGF-β-induced epithelial-mesenchymal transition and metastasis by controlling TIF1γ in non-small cell lung cancer. Mol Cancer. 2018;17(1):140. Published 2018 Sep 27. doi:10.1186/s12943-018-0889-7(IF:7.776)

[11] Cao X, Xu P, Liu Y, et al. Arabidopsis cryptochrome 1 promotes stomatal development through repression of AGB1 inhibition of SPEECHLESS DNA-binding activity. J Integr Plant Biol. 2021;63(11):1967-1981. doi:10.1111/jipb.13168(IF:7.061)

[12] Du SS, Li L, Li L, et al. Photoexcited Cryptochrome2 Interacts Directly with TOE1 and TOE2 in Flowering Regulation. Plant Physiol. 2020;184(1):487-505. doi:10.1104/pp.20.00486(IF:6.902)

[13] Zhou H, Qi Z, Pei P, et al. Biocompatible nanomicelles for sensitive detection and photodynamic therapy of early-stage cancer. Biomater Sci. 2021;9(18):6227-6235. Published 2021 Sep 14. doi:10.1039/d1bm00847a(IF:6.843)

[14] Zhang L, Zhou J, Yan Y, et al. Excipient-free nanodispersion of 7-ethyl-10-hydroxycamptothecin exerts potent therapeutic effects against pancreatic cancer cell lines and patient-derived xenografts. Cancer Lett. 2019;465:36-44. doi:10.1016/j.canlet.2019.08.019(IF:6.508)

[15] Geng Y, Duan H, Xu L, et al. BMP-2 and VEGF-A modRNAs in collagen scaffold synergistically drive bone repair through osteogenic and angiogenic pathways. Commun Biol. 2021;4(1):82. Published 2021 Jan 19. doi:10.1038/s42003-020-01606-9(IF:6.268)

[16] Qiu J , Peng P , Xin M , et al. ZBTB20-mediated titanium particle-induced peri-implant osteolysis by promoting macrophage inflammatory responses. Biomater Sci. 2020;8(11):3147-3163. doi:10.1039/d0bm00147c(IF:6.183)

[17] Zhou C, Wang G, Jing W, Tan X, Guo H. Anticancer Properties and Mechanisms of Singly-Protonated Dehydronorcantharidin Silver Coordination Polymer in a Bladder Cancer Model. Front Pharmacol. 2021;12:618668. Published 2021 Feb 23. doi:10.3389/fphar.2021.618668(IF:5.811)

[18] Sheng Y, Yu H, Pan H, et al. Genome-Wide Analysis of the Gene Structure, Expression and Protein Interactions of the Peach (Prunus persica) TIFY Gene Family. Front Plant Sci. 2022;13:792802. Published 2022 Feb 17. doi:10.3389/fpls.2022.792802(IF:5.754)

[19] Jia MZ, Liu LY, Geng C, Jiang J. Activation of 1-Aminocyclopropane-1-Carboxylic Acid Synthases Sets Stomatal Density and Clustered Ratio on Leaf Epidermis of Arabidopsis in Response to Drought. Front Plant Sci. 2021;12:758785. Published 2021 Dec 6. doi:10.3389/fpls.2021.758785(IF:5.754)

[20] Xuan Z, Zhao L, Li Z, et al. EPS8L3 promotes hepatocellular carcinoma proliferation and metastasis by modulating EGFR dimerization and internalization. Am J Cancer Res. 2020;10(1):60-77. Published 2020 Jan 1. (IF:5.177)

[21] Chen G, Fan XY, Zheng XP, Jin YL, Liu Y, Liu SC. Human umbilical cord-derived mesenchymal stem cells ameliorate insulin resistance via PTEN-mediated crosstalk between the PI3K/Akt and Erk/MAPKs signaling pathways in the skeletal muscles of db/db mice. Stem Cell Res Ther. 2020;11(1):401. Published 2020 Sep 16. doi:10.1186/s13287-020-01865-7(IF:5.116)

[22] Chen G, Fan XY, Zheng XP, Jin YL, Liu Y, Liu SC. Human umbilical cord-derived mesenchymal stem cells ameliorate insulin resistance via PTEN-mediated crosstalk between the PI3K/Akt and Erk/MAPKs signaling pathways in the skeletal muscles of db/db mice. Stem Cell Res Ther. 2020;11(1):401. Published 2020 Sep 16. doi:10.1186/s13287-020-01865-7(IF:5.116)

[23] You D, Du D, Zhao X, Li X, Ying M, Hu X. Mitochondrial malic enzyme 2 promotes breast cancer metastasis via stabilizing HIF-1α under hypoxia. Chin J Cancer Res. 2021;33(3):308-322. doi:10.21147/j.issn.1000-9604.2021.03.03(IF:5.087)

[24] Qin L, Zhao R, Chen D, et al. Chimeric antigen receptor T cells targeting PD-L1 suppress tumor growth. Biomark Res. 2020;8:19. Published 2020 Jun 3. doi:10.1186/s40364-020-00198-0(IF:4.866)

[25] Zuo X, Xiang W, Li K, et al. MdGRF11, a growth-regulating factor, participates in the regulation of flowering time and interacts with MdTFL1/MdFT1 in apple. Plant Sci. 2022;321:111339. doi:10.1016/j.plantsci.2022.111339(IF:4.729)

[26] Yang X, Yu Q, Xu H, Zhou J. Upregulation of CD22 by Chidamide promotes CAR T cells functionality. Sci Rep. 2021;11(1):20637. Published 2021 Oct 19. doi:10.1038/s41598-021-00227-4(IF:4.380)

[27] Mei Z, Zhang K, Lam AK, et al. MUC1 as a target for CAR-T therapy in head and neck squamous cell carinoma. Cancer Med. 2020;9(2):640-652. doi:10.1002/cam4.2733(IF:3.357)

[28] Zhang Z, Liu L, Cao S, Zhu Y, Mei Q. Gene delivery of TIPE2 inhibits breast cancer development and metastasis via CD8<sup>+</sup> T and NK cell-mediated antitumor responses. Mol Immunol. 2017;85:230-237. doi:10.1016/j.molimm.2017.03.007(IF:3.236)

[29] Yuan L, Zhou M, Huang D, et al. Resveratrol inhibits the invasion and metastasis of colon cancer through reversal of epithelial‑ mesenchymal transition via the AKT/GSK‑3β/Snail signaling pathway. Mol Med Rep. 2019;20(3):2783-2795. doi:10.3892/mmr.2019.10528(IF:2.952)

[30] Zhang C, Liu X, Chen F, et al. Gliotoxin destructs the pulmonary epithelium barrier function by reducing cofilin oligomer formation to promote the dissolution of actin stress fibers. Microb Pathog. 2018;123:169-176. doi:10.1016/j.micpath.2018.07.007(IF:2.332)

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