Recombinant Human IL-12 Protein _90111ES

YeasenSKU: 90111ES10

Size: 10 μg
Sale price$190.00

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Product Description


Interleukin-12 (IL-12) is a cytokine that has regulatory effects on T and natural killer (NK) cells and is composed of two disulfide-bonded subunits, p40 and p35. It is a heterodimeric cytokine produced by macrophages, mitogen stimulated- or EBV infected-B lymphocytes, keratinocytes, and probably dendritic cells, with important immunoregulatory functions in vitro and in vivo. In its role as the initiator of cell-mediated immunity, it has been suggested that IL-12 has therapeutic potential as a stimulator of cell-mediated immune responses to microbial pathogens, metastatic cancers, and viral infections such as AIDS. Importantly, IL-12 also inhibited secondary granuloma formation in mice presensitized with eggs demonstrating a role for the cytokine in reversing established Th2-type responses.


Product Properties



Interleukin-12, IL-12, NKSF2, CLMF p40






Sf21-derived human IL-12 protein.

Molecular Weight

Approximately 41 kDa and 29 kDa under reducing conditions.

AA Sequence

HuIL-12 p40: Ile23 - Ser328; Accession # P29460
HuIL-12 p35: Arg23 - Ser219; Accession # P29459



Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.


> 97 % by SDS-PAGE.

Biological Activity

Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. The ED50 for this effect is 0.01-0.05 ng/mL. The specific activity of rH IL-12 is approximately 1.1 × 104 units/μg, which is calibrated against rHuIL-12 WHO Standard (NIBSC code: 95/544).


< 1.0 EU per 1μg of the protein by the LAL method.


Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.2.


We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS to a concentration of 0.1 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20. Further dilutions should be made in appropriately buffered solutions.


Shipping and Storage


The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.



1. Avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For research use only!

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