The Murine RNase inhibitor is a soluble mouse-source recombinant protein expressed in E.coli. It can inhibit all kinds of RNase (RNase A, B, and C). The Murine RNase inhibitor is compatible with the Hifair® Ⅱ Reverse Transcriptase（Cat#：11110ES）, and any kinds of DNA Polymerases. Compared with the human-source RNase inhibitor, the Murine RNase inhibitor doesn’t contain the two Cys that is sensitive to oxidation, and therefore has a higher antioxidant activity and is more suitable for high-DTT-sensitive experiments (i.e. qPCR).
This product can be applied to 1st strand synthesis of cDNA, polysome isolation, in vitro reverse transcription and in vitro cellfree translation system.
Transport in ice packs，it can be stored at -20℃ for 2 years.
One activity unit (U) is defined as the enzyme needed for inhibiting 50% activity of 5 ng RNase A. The activity of RNase A is detected by quantifying the hydrolysis of Cyclic 2’, 3’-CMP to 3’-CMP.
Exonuclease Activity: Incubation of 200 U of this enzyme with 0.6 μg λ-Hind III at 74℃ for 1 hour results in no change on electrophoretic bands.
Endonuclease Activity: Incubation of 200 U of this enzyme with 0.6 μg Supercoiled pBR322 DNA for 1 hour at 74℃ results in no change on electrophoretic bands.
Residual E.coli gDNA Test: Detecting the residual nuclear acid in 200 U of this enzyme with 16S rDNA-specific qPCR, the genome DNA of is less than 10 copies.
Preparation of a first strand cDNA synthesis reaction mixture.
5× Hifair® Ⅱ Buffer
Oligo (dT)18 (50 μM)
dNTP Mix (10 mM each)
RNase Inhibitor (40 U/μL)
Hifair® Ⅱ Reverse Transcriptase (200 U/μL)
Total RNA:1 ng-5 μg
Mix gently with a pipette.
42℃ 45 min，85℃ 5 min.
The product store at -20℃.
The Murine RNase Inhibitor can inhibit RNase activity under a board spectrum of pH. The highest inhibitory activity is obtained at pH 7-8.
The activity of Murine RNase Inhibitor can be inactivated by bubbling or stirring intensely (i.e. Vortexing).
No inhibitory activity for RNase H.
For your safety and health, please wear experimental clothes and disposable gloves.