dsDNA HS (high sensitive) Assay Kit for Qubit® is a fast, sensitive and accurate double-stranded DNA (dsDNA) fluorescence quantitative detection kit. The kits include concentrated assay reagent, dilution buffer, and prediluted DNA standards. This kit is highly selective for dsDNA over RNA and is accurate for initial sample concentration from 10 pg/μL to 100 ng/μL. It is an ideal kit for DNA sample quantification (such as NGS input DNA quantification, DNA library quantification). Common contaminants such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay.
200×concentrate in DMSO
dsDNA Standard 1
0 ng/μL in TE buffer
dsDNA Standard 2
10 ng/μL in TE buffer
All the components can be stored at 2-8℃ for one year.
1. The operation flow of Qubit
1）Equilibrate the components in the kit to room temperature before use.
2）Set up the required number of 0.5-mL tubes for standards and samples. Do not label the side of the tube as this could interfere with the sample read. Label the lid of each standard tube correctly. Use only thin-wall, clear, 0.5-mL PCR tubes. Acceptable tubes include Qubit® assay tubes (Cat. No. Q32856) or Axygen® PCR-05-C tubes (Cat. No. 10011-830).
Prepare the working solution by diluting the dsDNA Reagent 1:200 in dsDNA Buffer. Use a clean plastic tube each time you prepare working solution. Do not mix the working solution in a glass container. Working solution is prepared for immediate use within 3 hous.
1）Add 10 µL of each dsDNA standard to the appropriate tube, then add working solution to individual assay tubes so that the final volume in each tube after adding sample is 200 µL. mix by vortexing 2–3 seconds. Be careful not to create bubbles.
2）Add 1-20 µL of each DNA samples or library to the appropriate tube, then add working solution to individual assay tubes so that the final volume in each tube after adding sample is 200 µL. mix by vortexing 2–3 seconds. Be careful not to create bubbles.
1）Incubate the tube protect from light at room temperature for 2 mins.
2）According to the operating instructions of the Qubit® Fluorometer, select dsDNA High Sensitivity as the assay type.
Table Effect of contaminants in the dsDNA HS Assay Kit for Qubit®
Concentration in 10-μL sample
Final concentration in the assay
Sodium dodecyl sulfate
Bovine serum albumin
*1× indicates a concentration equal to the concentration of dsDNA.