2× Hieff™ PCR Master Mix (With Dye) -10102ES

YeasenSKU: 10102ES03

Size: 1mL
Sale price$15.00

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2×Hieff™ PCR Master Mix contains Hieff™ Taq DNA Polymerase (Cat#10101), dNTPs, and other PCR-required components. The Master Mix is stable for 3 months at 4°C with our customized stabilizers. The pre-mix solution is optimized for conventional PCR and ready to use by adding DNA template and primers. The PCR products can be loaded directly for electrophoresis with pre-loaded bromophenol blue dye. The amplified products contain 3 '-dA protrusion and can be easily cloned into T vector. The 2×HieffTM PCR Master Mix simplifies PCR procedure and reduces contamination.


  • Convenient, ready-to-use mix
  • Thermostable—half-life is more than 40 min at 95°C
  • Generates PCR products with 3'-dA overhangs


  • Routine PCR amplification of DNA fragments
  • High throughput PCR
  • DNA labeling


Fidelity (vs. Taq) 1 ×
Hot Start No
Overhang 3'-A
Polymerase Taq DNA Polymerase
Reaction Format SuperMix or Master Mix
Reaction Speed Standard
Product Type PCR Master Mix (2×)


Components No. Name 10102ES03 10102ES08 10102ES50 10102ES60
10102 2×Hieff PCR Master Mix (With Dye) 1 mL 5×1 mL 50×1 mL 100×1 mL

Shipping and Storage

The 2×Hieff™ PCR Master Mix products should be stored at -15℃ ~ -25℃ for 2 years.


Figure1. The expected 1.2 kb PCR products can be amplified with 2×HieffTM PCR Master Mix.

The Master Mix was stored at -20°C for 1 year following another 3 months at 4°C and 1 month at 25°C. Template: Arabidopsis genome. Annealing temperature: 60°C. Extension time: 40 sec.


Cited from "Ultrasensitive sensors reveal the spatiotemporal landscape of lactate metabolism in physiology and disease" 

Cell Metab . 2023 Jan 3;35(1):200-211.e9. doi: 10.1016/j.cmet.2022.10.002

Citations & References:

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[12] Ullah H, Arbab S, Khan MIU, et al. Circulating cell-free mitochondrial DNA fragment: A possible marker for early detection of Schistosoma japonicum. Infect Genet Evol. 2021;88:104683. doi:10.1016/j.meegid.2020.104683(IF:2.773)

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