2× Hieff™ PCR Master Mix (No Dye) -10103ES

YeasenSKU: 10103ES03

Size: 1mL
Sale price$15.00

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2×Hieff™ PCR Master Mix is a kind of conventional PCR premixed solution which is ready to use, including Hieff™ Taq DNA Polymerase (Cat#10101), dNTP mix, MgCl2 and optimized buffer. During the reaction, only the primer and template can be added for amplification, which greatly simplifies the operation steps of experiment. 
This product contains excellent stabilizers and can be stored for 3 months at 4°C. The PCR product have 3 '-dA protrusion and can be easily cloned into T vector. 


  • Convenient, ready-to-use mix
  • Thermostable—half life is more than 40 min at 95°C
  • Generates PCR products with 3'-dA overhangs


  • Routine PCR amplification of DNA fragments
  • High throughput PCR


Fidelity (vs. Taq) 1 ×
Hot Start No
Overhang 3'-A
Polymerase Taq DNA Polymerase
Reaction Format SuperMix or Master Mix
Reaction Speed Standard
Product Type PCR Master Mix (2×)


Components No. Name 10103ES03 10103ES08 10103ES60
10103 2×Hieff™ PCR Master Mix (With Dye) 1 mL 5×1 mL  100×1 mL


The 2×Hieff™ PCR Master Mix products should be stored at -25°C~-15℃ for 2 years.


2×Hieff™ PCR master mix amplification result

Figure 1. The expected 1.2 kb PCR products can be amplified with 2×Hieff™ PCR Master Mix. 

The Master Mix was stored at-20°C for 1 year following another 3 months at 4°C and 1 month at 25°C. Template: Arabidopsis genome. Annealing temperature: 60°C. Extension time: 40 sec.

Citations & References:

[1] Liu Y, Lai M, Li S, et al. LS-106, a novel EGFR inhibitor targeting C797S, exhibits antitumor activities both in vitro and in vivo. Cancer Sci. 2022;113(2):709-720. doi:10.1111/cas.15229(IF:6.716)

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