50× ROX Reference Dye _ 10200ES

YeasenSKU: 10200ES60

Size: 100 μL
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Sale price$50.00

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Description

ROX Reference Dye is used in the Real Time PCR amplicator of ABI, Stratagene and other companies to correct for the interhole error caused by the addition. The product concentration is 25 µ M.

Product information

Cat.No

10200ES60 / 10200ES76 / 10200ES80

specifications

100 μL  / 500 μL  / 1 mL

Condition of storage

-25~ -15℃ divide up and save from light to avoid repeated freezing and thawing. Effective period 2 years. Short-term use can be used for 4℃.

Usage

1. 50× ROX Reference Dye

Quantitative system: add 1 L µ 50 ROX Reference Dye to the 50 µL qPCR system.

2. 50× ROX Reference Dye II

1)50 ROX Reference Dye II configuration Method: 10 times diluted in 50 ROX Reference Dye *.

 ROX dilution: 20 mM Tris-HCl (pH 8.4), 0.1 mM EDTA, 0.01% Tween 20.

2) Quantitative system: add 1 µ L 50 ROX Reference Dye II * * in 50 µL qPCR system.

* It can be diluted and added according to the system size; * * For the porous system preparation, it is recommended to prepare the premixed solution and mix it thoroughly before use.

Applicable instruments

50 ROX Reference Dye Suitable model:

ABI 5700, 7000, 7300, 7700, 7900HT Fast, StepOne , StepOne  Plus.

50 ROX Reference Dye II Suitable model:

 ABI 7500, 7500 Fast, ViiA7, QuantStudio  3 and 5, QuantStudio  6,7,12k Flex.

Stratagene  MX3000P, MX3005P, MX4000P.

 Instrument model without Rox correction:

 Bio-Rad CFX96, CFX384, iCycler  iQ , iQ  5, MyiQ , MiniOpticon , Opticon , Opticon  2, Chromo4.

 Roche Applied Science LightCycler  480, LightCycler  2.0; Lightcycler  96.

 Eppendorf Mastercycler  ep realplex , realplex  2 s.

 Qiagen Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000.

 Thermo  Scientific PikoReal  Cycler.

 Cepheid SmartCycler .

Illumina Eco qPCR.

Note

1. This product is used for scientific research purposes only.

2. For your safety and health, please wear laboratory clothes and wear disposable gloves.

 

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