Proteinase K -10401ES

YeasenSKU: 10401ES60

Size: 100mg
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Sale price$95.00

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In stock (49 units), ready to be shipped
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Description

Proteinase K is a serine protease with wide cleavage activity, which can cleave the carboxyl-terminal peptide bond of aliphatic and aromatic amino acids. Its relative molecular weight is about 29.3 kDa. Proteinase K is widely used in the preparation of chromosomal DNA for pulse electrophoresis, Western blotting, and the removal of a nuclease from DNA and RNA preparation. Denaturant such as SDS (1%) can improve its activity. In addition, the common working concentration is 50-100 μg/mL, and the specific working concentration is according to whether the buffer contains SDS, urea, pH, temperature, and other factors.

Applications

  • Genomic DNA extraction
  • Digestion and removal of the enzyme

Specifications

Source Yeast
Specific Activity ≥30 U/mg
Unit Definition One unit is defined as the amount of proteinase K that will liberate 1μmoL tyrosine per minute at 37℃ @ pH 7.5.
Purification Ni column affinity purification
Reaction Condition The reaction buffer should be incubated at 37℃ -70℃ for 1h, and the recommended reaction temperature is 70℃.
Inactivation method The reaction system can be inactivated by adding PMSF or DFP inhibitor and partially inactivated by incubation at 65℃ for 10-15 min.
Activity Range It has activity in a wide range of pH (4.0-12.0) and the optimal pH range is 7.5-9.0.
RNase and DNase None

Components

Components No. Name 10401ES60 10401ES80 10401ES90 10401ES98
10401 Proteinase K 100 mg 1 g 5 g 100 g

Shipping and Storage

The product is shipped with ice packs and can be stored at 2℃ ~ 8℃ for three years.

Citations & References:

[1] Li W, Qiu S, Chen J, et al. Chimeric Antigen Receptor Designed to Prevent Ubiquitination and Downregulation Showed Durable Antitumor Efficacy. Immunity. 2020;53(2):456-470.e6. doi:10.1016/j.immuni.2020.07.011(IF:22.553)

[2] Zhang Z, Mou Z, Xu C, et al. Autophagy-associated circular RNA hsa_circ_0007813 modulates human bladder cancer progression via hsa-miR-361-3p/IGF2R regulation. Cell Death Dis. 2021;12(8):778. Published 2021 Aug 7. doi:10.1038/s41419-021-04053-4(IF:8.469)

[3] Ni H, Qin H, Sun C, et al. MiR-375 reduces the stemness of gastric cancer cells through triggering ferroptosis. Stem Cell Res Ther. 2021;12(1):325. Published 2021 Jun 5. doi:10.1186/s13287-021-02394-7(IF:6.832)

[4] Chen S, Jia Z, Cai M, et al. SP1-Mediated Upregulation of Long Noncoding RNA ZFAS1 Involved in Non-syndromic Cleft Lip and Palate via Inactivating WNT/β-Catenin Signaling Pathway. Front Cell Dev Biol. 2021;9:662780. Published 2021 Jun 29. doi:10.3389/fcell.2021.662780(IF:6.684)

[5] Ma J, Chen S, Hao L, et al. Hypermethylation-mediated down-regulation of lncRNA TBX5-AS1:2 in Tetralogy of Fallot inhibits cell proliferation by reducing TBX5 expression. J Cell Mol Med. 2020;24(11):6472-6484. doi:10.1111/jcmm.15298(IF:4.486)

[6] Shi L, Gong Y, Zhuo L, Wang S, Chen S, Ke B. Methyltransferase-like 3 upregulation is involved in the chemoresistance of non-small cell lung cancer. Ann Transl Med. 2022;10(3):139. doi:10.21037/atm-21-6608(IF:3.932)

[7] Ma J, Chen S, Hao L, et al. Long non-coding RNA SAP30-2:1 is downregulated in congenital heart disease and regulates cell proliferation by targeting HAND2. Front Med. 2021;15(1):91-100. doi:10.1007/s11684-020-0778-5(IF:3.297)

[8] Huang Q, Xie Y, Yang Z, et al. The cytoplasmic loops of AgrC contribute to the quorum-sensing activity of Staphylococcus aureus. J Microbiol. 2021;59(1):92-100. doi:10.1007/s12275-021-0274-x(IF:2.845)

[9] Guo T, Xing Y, Chen Z, et al. Core-binding factor beta is required for osteoblast differentiation during fibula fracture healing. J Orthop Surg Res. 2021;16(1):313. Published 2021 May 14. doi:10.1186/s13018-021-02410-9(IF:2.359)

[10] Zhang W, Zhang T, Yan Y, et al. Exosomal miR-22-3p Derived from Chronic Rhinosinusitis with Nasal Polyps Regulates Vascular Permeability by Targeting VE-Cadherin. Biomed Res Int. 2020;2020:1237678. Published 2020 Nov 12. doi:10.1155/2020/1237678(IF:2.276)

[11] Cheng L, Yi X, Shi Y, et al. Abnormal lipid metabolism induced apoptosis of spermatogenic cells by increasing testicular HSP60 protein expression. Andrologia. 2020;52(11):e13781. doi:10.1111/and.13781(IF:1.951)

[12] Zhang T, Zhou Y, You B, et al. miR-30a-5p Inhibits Epithelial-to-Mesenchymal Transition by Targeting CDK6 in Nasal Polyps. Am J Rhinol Allergy. 2021;35(2):152-163. doi:10.1177/1945892420939814(IF:1.943)

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