CleaScrip™ T7 RNA Polymerase (low dsRNA, 250 U/μL) -10628ES

YeasenSKU: 10628ES10

Size: 10 KU
Price:
Sale price$180.00

Shipping calculated at checkout

Stock:
In stock (12 units), ready to be shipped
Bulk Quote Inquiry

Description

This is an engineered T7 RNA polymerase variant (low dsRNA) derived from the wild-type T7 RNA polymerase and produced in Escherichia coli. It significantly reduces the production of double-stranded RNA (dsRNA) while efficiently incorporating cap analogs, and exhibiting highly efficient in vitro transcription (IVT) comparable to the wild-type T7 RNA polymerase. It catalyzes the 5'→3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates.

Note: G* is the first base of the RNA transcript.

Feature

  • dsRNA level lower to about 1/100000
  • Comptiable with Trilink CleanCap AG
  • High yields comparable to WT 
  • Lower Cap input
  • Animal origin-free (AOF)

Please find information on the development of this enzyme.

Components

Components No.

Name

10628ES10

10628ES60

10628ES72

10628ES86

 

 

(10 KU)

(100 KU)

(250 KU)

(2,500 KU)

10628

CleaScrip™ T7 RNA Polymerase (low dsRNA, 250 U/μL)

40 μL

400 μL

1 mL

10 mL

Specifications 

Source

Recombinant E. coli with T7 RNA Polymerase gene

Optimum Temperature

37℃

Storage Buffer

50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% Triton X-100,50% (v/v) glycerin,pH7.9 at 25℃

Unit Definition

The amount of enzyme required to incorporate 1 nmol of [3H] GMP into the acid-insoluble precipitate within 1 hour at 37°C and pH 8.0 is defined as 1 unit.

 

QC Standard

Items

Specification/Standard

Enzyme activity

250-300U/μL

Protein Purity

≥95%

Endotoxin

<20 EU/mg

Protease

Negative

Exonuclease

Negative

Nickase

Negative

RNase

Negative

E.coli host protein

<50ppm

E.coli host DNA

<10 fg/U

Mycoplasma examination

Negative

Related Products

Tris NTPs synergistically decreasing dsRNA



Figures

Customer feedback:
Figure 1. Testing of T7 RNA Polymerase mutants. dsRNA Content: The dsRNA content reduced by more than 10-fold (A).  mRNA Integrity: Maintained at over 90% (B).  2K Fragment Capping Efficiency: Exceeded 99% (C, D).


Table 1. The Yields of low dsRNA T7 RNA polymerase is comparable with that of WT.

Shipping and Storage

The products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.

Documents

MSDS

    You may also like

    Inquiry