Uracil DNA Glycosylase (UDG), Heat-Labile (Glycerol-Free) (1 U/μL) -10707ES

YeasenSKU: 10707ES76

Size: 500 U
Price:
Sale price$395.00

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Description

UDG (uracil DNA glycosylase) can catalyze the hydrolysis of the N-glycosidic link between the uracil base and the sugar-phosphate backbone in ssDNA and dsDNA. It can easily control aerosol pollution and is suitable for common molecular biology systems such as PCR, qPCR, RT-qPCR and LAMP.  Compared to regular UDG, Heat-Labile UDG avoids the potential residual activity that regular UDG may have after inactivation, which could degrade dU-containing amplification products at room temperature. 

 Applications

1. Remove aerosol contamination from PCR products containing dU.

2. Remove single-stranded or double-stranded DNA uracil bases.

Specifications

Unit Definition

The amount of enzyme required to completely degrade 1 μg of dsDNA containing uracil within 30 minutes at 25°C is defined as 1 activity unit (U).

Deactivation method

94℃, 2-5 minutes.


Components


No.

Name

10707ES60

(100 U)

10707ES76

(500 U)

10707ES90

(5 KU)

10707ES92

(10 KU)

10707

Uracil DNA Glycosylase (UDG, Glyceol-free), (1 U/μL)

100 μL

500 μL

5 mL

10 mL



Shipping and Storage

Ice pack transport. Store at 4°C, valid for 6 months.


Precautions


1. For your safety and health, please wear a lab coat and disposable gloves.

2. Thermosensitive UDG is active in most PCR reaction buffers.
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