Description
UDG (uracil DNA glycosylase) can catalyze the hydrolysis of the N-glycosidic link between the uracil base and the sugar-phosphate backbone in ssDNA and dsDNA. It can easily control aerosol pollution and is suitable for common molecular biology systems such as PCR, qPCR, RT-qPCR and LAMP. Compared to regular UDG, Heat-Labile UDG avoids the potential residual activity that regular UDG may have after inactivation, which could degrade dU-containing amplification products at room temperature.
Applications
1. Remove aerosol contamination from PCR products containing dU.
2. Remove single-stranded or double-stranded DNA uracil bases.
Specifications
|
Unit Definition |
The amount of enzyme required to completely degrade 1 μg of dsDNA containing uracil within 30 minutes at 25°C is defined as 1 activity unit (U). |
|
Deactivation method |
94℃, 2-5 minutes. |
Components
|
No. |
Name |
10707ES60 (100 U) |
10707ES76 (500 U) |
10707ES90 (5 KU) |
10707ES92 (10 KU) |
|
10707 |
Uracil DNA Glycosylase (UDG, Glyceol-free), (1 U/μL) |
100 μL |
500 μL |
5 mL |
10 mL |
Shipping and Storage
Ice pack transport. Store at 4°C, valid for 6 months.Precautions
1. For your safety and health, please wear a lab coat and disposable gloves.
2. Thermosensitive UDG is active in most PCR reaction buffers.