Hieff Unicon™ Universal TaqMan multiplex qPCR master mix -11211ES

YeasenSKU: 11211ES03

Size: 100 T
Price:
Sale price$50.00

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Stock:
In stock (16 units), ready to be shipped
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Description

Hieff Unicon™ Universal Blue qPCR Master Mix (Probe Based) is a pre-solution for 2×real-time quantitative PCR amplification characterized by high sensitivity and specificity, is blue in color, and has the effect of sample addition

This product is a 2× Mix pre-mixed reagent that enables up to four fluorescent quantitative PCR reactions in a single reaction well. This product contains the genetically modified antibody method to hot-start Taq enzyme, greatly improving the amplification sensitivity and specificity. At the same time, this product has deeply optimized the multi-reaction buffer, which can improve the amplification efficiency of the reaction and promote the effective amplification of low-concentration templates. This product can be used for genotyping and multiplex quantitative analysis.

Features

  • Strong tolerance of blood impurities
  • High sensitivity
  • Super storage stability

Applications

  • Pathogen detection
  • Copy number analysis
  • SNP genotyping

Specifications

Hot Start Built-in hot start
Detection method Primer-probe detection
PCR method qPCR
Polymerase Taq DNA polymerase
Type of sample DNA
Application equipment Applied Biosystems: 5700, 7000, 7300, 7700, 7900HT Fast, StepOne™, StepOne Plus™, 7500, 7500 Fast, ViiA™7, QuantStudio™ 3 and 5, QuantStudio™ 6,7,12k Flex; Bio-Rad: CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4; Eppendorf: Mastercycler ep realplex, realplex 2 s; Qiagen: Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000; Roche Applied Science: LightCycler 480, LightCycler 2.0; Lightcycler 96; Stratagene: MX3000P™, MX3005P™, MX4000P™; Thermo Scientific: PikoReal Cycler; Cepheid: SmartCycler; Illumina: Eco qPCR.  

Components

No. Name

11211ES03

100T

11211ES08

500T

11211ES20

2,000T

11211ES61

10,000T

11211

Hieff Unicon™Universal Multiplex

qPCR Master Mix (Probe Based)

1 mL 5×1 mL 20 mL 100 mL


PCR Reaction setup

Components

Volume (μL)

Volume (μL)

Final Concentration

2× Hieff UniconTM Universal TaqMan 

multiplex qPCR master mix

10

12.5

Primer mix (10 μmol/L)

x

x

0.1-0.5 μmol/L

Probe mix (10 μmol/L)

x

x

50-250 nmol/L

Rox reference dye

0.4

0.4

Template DNA/cDNA

1-10

1-10

-

ddH2O

up to 20

up to 25

-

Rox reference dye is optional

Cycle Setup

CYCLE STEP
TEMPERATURE
 TIME  CYCLES
 Initial Denaturation
 95°C  5 mins
 1
 Denaturation

 Extension
 95°C

 60°C
 15 secs

 30 secs

 40-45


Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 2 year.

Figures

Figure 1 High stability: 37℃ thermal acceleration stability

The red line: 37℃ thermal acceleration 0 day; The yellow line: 37℃ thermal acceleration 7 days; The blue line: 37℃ thermal acceleration 14 days

Figure 2 High stability: freeze-thaw stability

The red line: freeze thaw o time; The yellow line: freeze thaw 50 times; The blue line: NTC

Citations & References:

[1] Zhang P, Lu S, Liu Z, et al. Transcriptomic and Metabolomic Profiling Reveals the Effect of LED Light Quality on Fruit Ripening and Anthocyanin Accumulation in Cabernet Sauvignon Grape. Front Nutr. 2021;8:790697. Published 2021 Dec 14. doi:10.3389/fnut.2021.790697(IF:6.576)

[2] Xu B, Zhang C, Jiang A, et al. Histone methyltransferase Dot1L recruits O-GlcNAc transferase to target chromatin sites to regulate histone O-GlcNAcylation. J Biol Chem. 2022;298(7):102115. doi:10.1016/j.jbc.2022.102115(IF:5.157)

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