Hieff NGS™ Fast-Pace DNA Cyclization Kit for MGI -13341ES

YeasenSKU: 13341ES96

Size: 96T
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Sale price$275.00

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Description


Hieff NGS™ Fast-Pace DNA Cyclization Kit for MGI is a single-strand cyclization kit specifically designed for MGI platforms. The use of high-quality enzymes and optimized Buffer significantly reaction efficiency, enabling the entire cyclization and digestion process to be completed in less than 30 minutes. This kit is suitable for all standard single-barcode libraries for MGI platforms and is not limited to different MGI sequencing platforms except for the limitations of the library-prep reagents.

Features

  • Wide range of use: compatible with mainstream library preparation kits (KAPA, etc.)
  • Fast cyclization: a 1-hour rapid cyclization
  • High cyclization efficiency: the cfDNA cyclization efficiency>30%,other sample types>40%

Applications

  • To prepare a single-stranded circular DNA library dedicated to the MGI high-throughput sequencer

Specifications

Product Type NGS Library Preparation
Product Line DNA library Preparation
Input DNA The recommended Input DNA is 1 pmol, if the PCR product is insufficient, down to 0.5 pmol
For Use With (Application) Single Barcode DNA Cyclization Kit for MGI
For Use With (Equipment) MGI Platforms
Quantity 16/96 Reactions

Components

Components No. Name 13341ES16 (16T) 13341ES96 (96T)
13341-A Splint Oligo 96 μL 576 μL
13341-B Splint Buffer 240 μL 2×720 μL
13341-C Ligase 80 μL 480 μL
13341-D Digestion Buffer 128 μL 768 μL
13341-E Digestion Enzyme 32 μL 192 μL

Storage

-25℃ ~ -15℃ storage, valid for one year.

Figures

Figure 1. Comparison of cyclization efficiency of PCR-free library preparation for different fragment lengths.

Method: The PCR-free libraries were prepared with Cat#12202 PCR-free library prep kit.
Results: The library cyclization efficiency of the different library fragments Cat#13341 was higher than that of Supplier M*

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