Description
TelN Protelomerase is a recombinant expression from phage N15. It cuts double-stranded DNA (dsDNA) at TelN recognition sequences (56 bp), and generates covalently closed ends at the cleavage sites, which can be applied to enzymatic synthesis of DNA.
Specifications
Components
|
Components No. |
Name |
14540ES72 |
14540ES80 |
14540ES90 |
|
14540-A |
TelN Protelomerase (5 U/μL) |
50 μL |
200 μL |
1 mL |
|
14540-B |
10 X TelN Reaction Buffer |
250 μL |
1 mL |
5 X 1 mL |
Storage
This product should be stored at -25~-15℃ for 1 years.
Instructions
1. Reaction system preparation
|
Components |
Volume (μL) |
|
dsDNA (< 300 fmol of TelN sites) |
X |
|
10 X TelN Reaction Buffer |
2 |
|
TelN Protelomerase (5 U/μL) |
1 |
|
Nuclease-free water |
To 20 |
2. Reaction condition: 30℃ for 30 min after mixing well.
3. Heat inactivation: 75℃ for 5 min.
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves，for your safety.
