Description
Description
MolPure® Cell /Tissue Total RNA Kit was performed by using MolPure® DNA removing / RNA binding column technology and new solution systems suitable for extracting high purity and quality total RNA from a variety of fresh or frozen animal tissues or cultured cells. The extraction process does not require toxic phenol, chloroform, β -mercaptoethanol, or precipitation of isopropanol and ethanol. Easy to operate, 15 min to complete the animal tissue or cells (10-30 mg animal tissue or (1-10) ×106 Animal cells) of total RNA extraction. The total RNA is of high purity and can be used in various molecular biology experiments including RT-PCR, qPCR, molecular cloning and RNase protection analysis.
Components
| No. | Name | 19221ES50 (50 T) |
| 19221-A | MolPure® DNA removing/RNA binding Column A | 100 |
| 19221-B | 2 mL Collection Tube A2 | 100 |
| 19221-C | Lysate buffer LB（LB Buffer A2） | 30 mL |
| 19221-D | Deproteinized buffer PL (PL Buffer A 2) | 40 mL |
| 19221-E | Binding buffer BD*(BD Buffer A 2*) | 10 mL |
| 19221-F | Wash Buffer W*(Wash Buffer A 2*) | 13 mL |
| 19221-G | RNase-free H2O | 5 mL |
Shipping and Storage
This product should be Store at room temperature, protected from light, for 24 months. 2-8℃lasts longer.
Precautions
Avoid exposing the reagent to air for a long time, which may cause volatilization, oxidation, and changes in pH value. After using each solution, tighten the lid in time.
Observe whether there is precipitation or turbidity in each solution (especially in low-temperature environments such as winter at room temperature), and heat at 37℃ to redissolve the solution to avoid affecting the use effect.
For your safety and health, please wear a lab coat and disposable gloves to operate.
This product is for research use only!
Published paper
[1] Ban Y, Chu Y, Pan F, Guo Z, Yang Y, Wei X, et al. Lipid‐based Nanocarriers Enabled Oral Delivery of Oleanolic Acid Derivative DKS26 for Diabetes Management. Advanced Healthcare Materials. (2023)
[2] Zhan, Z., Liu, W., Pan, L. et al. Overabundance of Veillonella parvula promotes intestinal inflammation by activating macrophages via LPS-TLR4 pathway. Cell Death Discov. 8, 251 (2022).