Description
Interleukin 19 (IL-19) is a member of the IL-10 family of cytokines. The IL-10 family is a class II alpha -helical collection of cytokines that contains two groups, a viral homolog and a cellular homolog group. Within the cellular homolog group, there are two additional groupings, one which uses IL-10 R2 as a signal transducing receptor (IL-10, IL-22 and IL-26), and one which uses IL-20 R2 as a signal transducing receptor (IL-19, IL-20 and IL-24). Mouse IL-19 is synthesized as a 176 amino acid (aa) precursor that contains a 24 aa signal sequence and a 152 aa mature region. Based on human studies, it is expected to be secreted as a glycosylated monomer, 35 - 45 kDa in size. IL-19 is unusual in that it contains seven amphipathic helices. Mature mouse IL-19 shares 69% aa sequence identity with the mature human IL-19, and 85% and 68% aa identity to unpublished Genbank sequences for rat and canine IL-19, respectively. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells. IL-19 expression is limited to activated keratinocytes and monocytes, with a possible contribution from B cells. IL-19 binds a receptor complex consisting of the IL-20 receptor alpha (also known as IL-20 R1) and the IL-20 receptor beta (IL-20 R2). This receptor complex is also shared by IL-20 and IL-24. Notably, IL-19 is reported to actually bind to IL-20 R2, which is generally considered to be only the signal transducing receptor subunit. Functionally, it has been reported that IL-19 both will and will not induce IL-6 and TNF production by monocytes. It does, however, seem to drive T-helper cell differentiation towards a Th2 response, inducing both IL-10 and production of itself .
Product Properties
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Synonyms |
IL-10C; IL19; MDA1; |
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Accession |
Q8CJ70 |
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GeneID |
329244 |
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Source |
E.coli-derived Mouse IL-19, Leu25-Ala176. |
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Molecular Weight |
Approximately 17.6 kDa. |
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AA Sequence |
LRRCLISVDM RLIEKSFHEI KRAMQTKDTF KNVTILSLEN LRSIKPGDVC CMTNNLLTFY RDRVFQDHQE RSLEVLRRIS SIANSFLCVQ KSLERCQVHR QCNCSQEATN ATRIIHDNYN QLEVSSAALK SLGELNILLA WIDRNHLETP AA |
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Tag |
None |
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Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Purity |
> 97% by SDS-PAGE and HPLC analyses. |
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Biological Activity |
The ED50 as determined by a cell proliferation assay using human IL-20Rα and human IL-20Rβ co-transfected murine BaF3 pro-B cells is less than 0.8 ng/mL, corresponding to a specific activity of > 1.25 × 106 IU/mg. Fully biologically active when compared to standard. |
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Endotoxin |
< 0.1 EU per 1μg of the protein by the LAL method. |
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Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, with 3 % Trehalose. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!