Description
C–X–C motif chemokine 10 (CXCL10) also known as interferon γ-induced protein 10kDa (IP-10) or small-inducible cytokine B10 is a cytokine belonging to the CXC chemokine family. The rhesus macaque IP-10 shares 69 %, 95 %, 74 % a.a. sequence identity with human, mouse, rat IP-10. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3. CXCL10 has been shown to be a chemoattractant for activated Tlymphocytes. CXCL10 has been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent antitumor effect. CXCL10 is also recognized as a biomarker that predicts severity of various diseases.
Product Properties
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Synonyms |
Gamma-IP10, Small-inducible Cytokine B10, Protein Mob-1 |
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Accession |
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GeneID |
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Source |
E.coli-derived rat IP-10/CXCL10 protein, Ile22-Pro98. |
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Molecular Weight |
Approximately 8.7 kDa. |
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AA Sequence |
IPLARTVRCT CIDFHEQPLR PRAIGKLEII PASLSCPHVE IIATMKKNNE KRCLNPESEA IKSLLKAVSQ RRSKRAP |
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Tag |
None |
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Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Purity |
>95% by SDS-PAGE and HPLC analyses. |
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Biological Activity |
The biological activity determined by a chemotaxis bioassay using human CXCR3 transfected HEK293 cells is in a concentration range of 10-50 ng/ml. Fully biologically active when compared to standard. |
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Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
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Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!