Description
Mouse C10 belongs to the CC chemokine family and is expressed in myelopoietic bone marrow cultures when stimulated with GM-CSF, M-CSF, IL-3 or IL-4. It signals primarily through the CCR1 receptor. C10 is chemotactic for B cells, CD4+ T cells, monocytes and NK cells and also exhibits powerful suppressive activity on colony formation by different lineages of hematopoietic progenitors. The C10 contains the four highly conserved cysteine residues present in CC chemokines. The mature protein contains 95 amino acid residues. Recombinant Mouse C10 is a 10.7 kDa protein containing 95 amino acid residues.
Product Properties
|
Synonyms |
Small-inducible Cytokine A6 |
|
Accession |
P27784 |
|
GeneID |
20305 |
|
Source |
E.coli-derived Rhesus Macaque Eotaxin protein,Gly22-Ala116. |
|
Molecular Weight |
Approximately 10.7 kDa. |
|
AA Sequence |
GLIQEIEKED RRYNPPIIHQ GFQDTSSDCC FSYATQIPCK RFIYYFPTSG GCIKPGIIFI SRRGTQVCAD PSDRRVQRCL STLKQGPRSG NKVIA |
|
Tag |
None |
|
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
Purity |
> 97% by SDS-PAGE and HPLC analyses. |
|
Biological Activity |
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human CCR1 transfected murine BaF3 cells is in a concentration range of 10-100 ng/mL |
|
Endotoxin |
<1 EU/μg of protein as determined by LAL method. |
|
Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, pH 8.0, 500 mM NaCl. |
|
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only.