Description
Description
GM-CSF is a powerful growth and differentiation factor which acts on hematopoietic progenitor cells and also activates differentiated granulocytes and macrophages.
Product information
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Synonyms |
Granulocyte-Macrophage Colony-Stimulating Factor, GM-CSF, CSF-2, MGI-1GM, Pluripoietin-α |
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Uniprot No. |
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Source |
Recombinant Mouse GM-CSF Protein is expressed from Yeast with N-HIS. It contains Ala18-Lys141. |
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Molecular Weight |
The recombinant mouse GM-CSF comprises 124 amino acids and has a predicted molecular mass of 14.1 kDa. |
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Purity |
> 95% as determined by SDS-PAGE. |
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Endotoxin |
<1 EU per 1μg of the protein by the LAL method. |
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Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4,with 1%BSA. |
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Reconstitution |
1. We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS, pH 7.4, with 0.1% BSA to a concentration of 0.1-1.0 mg/mL. 2. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. 3. Further dilutions should be made in PBS, which must contain carrier proteins, such as 0.1% BSA, 10% FBS, 5% HSA, 5% trehalose, one of four options. 5% trehalose is recommended for experimental culture without serum. |
Storage
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Notes
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.