Recombinant Human TGF-alpha Protein _91704ES

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Catalog ID: 91704ES60
Size: 100 μg
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Description

Transforming Growth Factor-alpha (TGF-α) , also known as sarcoma growth factor, TGF-type I and ETGF, is a member of the EGF family of cytokines.  TGF-α is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells.Mature TGF-alpha  shows approximately 93% amino acid sequence identity with mouse or rat TGF-alpha and is not species specific in its biological effects.

 

Product Properties

Synonyms

Transforming Growth Factor alpha; TGF-a; TGF-type I; ETGF

Accession

P01135

Gene ID

7039

Source

E.coli-derived Human TGF-a protein, Vla40-Ala89 

Molecular Weight

Approximately 5.8 kDa, on SDS-PAGE under reducing conditions.

AA Sequence

V VSHFNDCPDS HTQFCFHGTC RFLVQEDKPA CVCHSGYVGA RCEHADLLA

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 95% as determined by SDS-PAGE

Biological Activity

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is < 0.4 ng/mL.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized after extensive dialysis against PBS.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -80 for 1 year from date of receipt.
1week, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 °C under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1.For your safety and health, please wear lab coats and disposable gloves for operation.

2.For research use only!

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