Description
Growth Differentiation Factor-5 (GDF-5; also called BMP-14 and CDMP-1) is a member of the BMP family of TGF-beta superfamily proteins. Human GDF-5, -6, and -7 are a defined subgroup of the BMP family. GDF-5 is synthesized as a homodimeric precursor protein consisting of a 354 amino acid (aa) N-terminal pro-region and a 120 aa C-terminal mature peptide. Mature human GDF-5 shares 99% aa sequence identity with both mature mouse and rat GDF-5. GDF-5 signaling is mediated by formation of a heterodimeric complex consisting of a type 1 (BMPR-IB) and a type II (BMPR-II or Activin RII) serine/threonine kinase receptor which results in the phosphorylation and activation of cytosolic Smad proteins (Smad1, 5, and 8). Similar to other BMP family proteins, GDF-5 signaling is antagonized by Noggin. GDF-5 is involved in multiple developmental processes including limb generation, cartilage development, joint formation, bone morphogenesis, cell survival, and neuritogenesis.
Product Properties
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Synonyms |
CDMP-1, CDMP1LAP4 |
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Accession |
P43026 |
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GeneID |
8200 |
|
Source |
E.coli-derived Human GDF-5/BMP-14, Ala382-Arg501. |
|
Molecular Weight |
Approximately 27.1 kDa. |
|
AA Sequence |
APLATRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR |
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Tag |
None |
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Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Purity |
> 95% by SDS-PAGE and HPLC analyses. |
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Biological Activity |
The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0 μg/mL, corresponding to a specific activity of > 1000 IU/mg. Fully biologically active when compared to standard. |
|
Endotoxin |
< 0.1 EU per 1μg of the protein by the LAL method. |
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Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in 30% Acetonitrile and 0.1% TFA. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1-1.5 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!