Recombinant Mouse Growth Differentiation Factor 5/Bone Morphogenetic Protein-14 (Mouse GDF-5/BMP-14) _92005ES

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Catalog ID: 92005ES10
Size: 10 μg
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Sale price$78.00

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Description

Growth Differentiation Factor-5 (GDF-5; also called BMP-14 and CDMP-1) is a member of the BMP family of TGF-beta superfamily proteins. Mature mouse GDF-5 shares 99% aa sequence identity with both mature human and rat GDF-5. Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems. GDF-5 is involved in multiple developmental processes including limb generation, cartilage development, joint formation, bone morphogenesis, cell survival, and neuritogenesis.

 

Product Properties

Synonyms

CDMP-1, GDF5, OS5, radotermin, SYNS2

Accession

P43027

GeneID

14563

Source

E.coli-derived mouse GDF-5/BMP-14 protein, Ala376-Arg495.

Molecular Weight

Approximately 27.2 kDa.

AA Sequence

APLANRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

>96% by SDS-PAGE and HPLC analyses.

Biological Activity

The ED50 as determined by inducing alkaline phosphatase production of mouse ATDC5 cells is less than 1.0 μg/ml, corresponding to a specific activity of > 1000 IU/mg. Fully biologically active when compared to standard.

Endotoxin

< 0.1 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 30% Acetonitrile and 0.1% TFA.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions 

1. Avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For research use only!

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