Recombinant Human LR3 IGF-1 Protein _92210ES

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Catalog ID: 92210ES80
Size: 1 mg
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Description

Long R3 IGF-I (LR3 IGF-I) is a 9.2 kDa synthetic analog of IGF-I that is generated by modifying the aa sequence for mature human IGF-I. The LR3IGF-1 is a long-term analog of human IGF-1, specifically designed and manufactured for mammalian cell culture to support large-scale manufacturing of recombinant biopharmaceuticals. LR3 IGF-I, an analogue of insulin-like growth factor (IGF)-I, was used in biopharmaceutical protein production in mammalian cells.

 

Product Properties

 

Synonyms

LR3 IGF-I, IBP1, IGF1, IGF-1, IGF1A, IGFI, IGF-I, IGF-IA, IGF-IB, MGF

Accession

P05019

GeneID

3479

Source

E.coli-derived human LR3 IGF-1 protein, Gly49-Ala118.

Molecular Weight

Approximately 9.1 kDa.

AA Sequence

MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

>96% by SDS-PAGE analyses.  

Biological Activity

1.Fully biologically active when compared to standard. Measured in a serum-free cell proliferation assay using human MCF-7 cells. The ED50 for this effect is 0.3-1.5 ng/mL, corresponding to a specific activity of > 6.7 × 105 U/mg.

2.The ED50 as determined by the stimulation of protein synthesis using rat L6 myoblasts is less than 10 ng/mL, corresponding to a specific activity of > 1.0 × 105 U/mg. Fully biologically active when compared to standard.

Endotoxin

0.01 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.2.

Reconstitution

Before use this product, please read the direction below carefully.

1.This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.

2.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.

Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

 

The product should be stored at -25~-15℃ for 1 year from date of receipt.

1 month, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~-15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

 

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

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