Description
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-3 (stromelysin-1), can degrade a broad range of substrates including collagen alpha chains, aggrecan, laminin, fibronectin, elastin, casein, alpha -1 antitrypsin, myelin basic protein, IL-1 beta, IGFBP-3, pro MMP-1, pro MMP-7, pro MMP-8, pro MMP-9 and pro MMP-13. The MMP-3 substrate repertoire extends beyond extracellular matrix proteins and implicates MMP-3 in roles other than direct tissue remodelling, for instance, enzyme cascades and cytokine regulation.
Product Properties
|
Synonyms |
Matrix Metalloproteinase-3, Stromelysin-1, SL-1, Transin-1 |
|
Uniprot No. |
P08254 |
|
Source |
Recombinant Human MMP-3 Protein is expressed from E.coli with His tag at the C-terminal. It contains Arg101-Cys477. |
|
Molecular Weight |
Approximately 42.8 kDa. |
|
Purity |
> 95% as determined by SDS-PAGE. |
|
Endotoxin |
<1 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
|
Reconstitution |
Centrifuge tubes before opening. The concentration of protein solution used for lyophilization is generally 1mg/mL. Dissolve lyophilized protein with sterile water to ensure the concentration is greater than 100 ug/mL. |
Shipping and Storage
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
Data
